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ai139 transgene  (Addgene inc)


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    Structured Review

    Addgene inc ai139 transgene
    Ai139 Transgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ai139+transgene/Ai139+(TIT2L-GFP-ICL-TPT)+targeting+vector+(Plasmid+%23114426)/pmc08985214-612-25-27
    Average 93 stars, based on 1 article reviews
    ai139 transgene - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transgenic Assay:

    Article Title: p21 produces a bioactive secretome that places stressed cells under immunosurveillance
    Article Snippet: .. To generate iL-p21 transgenic mice on a pure C57BL/6 background, the following targeting construct was cloned: pTRE2-LoxP-STOP-LoxP(LSL)-p21-Myc-Flag-WPRE-pA using the pTRE2 promoter and LSL from the Ai139 transgene (Addgene, #114426) and p21-Myc-Flag from the L-p21 transgene (Origene, #MR227529) as described above. .. Homology arms spanning 968 bp at the 5′-end and 937 bp at the 3′-end flanked by sgRNA target sites were used to target the construct into the Col1a1 locus of C57BL/6NHsd (Envigo) zygotes using CRISPR-Cas9-mediated gene editing with Cas9 mRNA (Trilink Biotechnologies, #L-7606) and Col1a1 -specific sgRNA 5′-GAGGTTCATGAGCCCTCAAA-3′.

    Article Title: p21 produces a bioactive secretome that places stressed cells under immunosurveillance.
    Article Snippet: .. To generate iL-p21 transgenic mice on a pure C57BL/6 background, the following targeting construct was cloned: pTRE2-LoxP-STOP-LoxP(LSL)-p21Myc-Flag-WPRE-pA using the TRE2 promoter and LSL from the Ai139 transgene (Addgene, #114426) and p21-Myc-Flag from the L-p21 transgene (Origene, #MR227529) as described above. .. Homology arms spanning 968 bp at the 5′-end and 937 bp at the 3′-end flanked by sgRNA target sites were used to target the construct into the Col1a1 locus of C57BL/6NHsd (Envigo) zygotes using CRISPR-Cas9-mediated gene editing with Cas9 mRNA (Trilink Biotechnologies, #L-7606) and Col1a1-specific sgRNA 5′-GAGGTTCATGAGCCCTCAAA-3′.

    Construct:

    Article Title: p21 produces a bioactive secretome that places stressed cells under immunosurveillance
    Article Snippet: .. To generate iL-p21 transgenic mice on a pure C57BL/6 background, the following targeting construct was cloned: pTRE2-LoxP-STOP-LoxP(LSL)-p21-Myc-Flag-WPRE-pA using the pTRE2 promoter and LSL from the Ai139 transgene (Addgene, #114426) and p21-Myc-Flag from the L-p21 transgene (Origene, #MR227529) as described above. .. Homology arms spanning 968 bp at the 5′-end and 937 bp at the 3′-end flanked by sgRNA target sites were used to target the construct into the Col1a1 locus of C57BL/6NHsd (Envigo) zygotes using CRISPR-Cas9-mediated gene editing with Cas9 mRNA (Trilink Biotechnologies, #L-7606) and Col1a1 -specific sgRNA 5′-GAGGTTCATGAGCCCTCAAA-3′.

    Article Title: p21 produces a bioactive secretome that places stressed cells under immunosurveillance.
    Article Snippet: .. To generate iL-p21 transgenic mice on a pure C57BL/6 background, the following targeting construct was cloned: pTRE2-LoxP-STOP-LoxP(LSL)-p21Myc-Flag-WPRE-pA using the TRE2 promoter and LSL from the Ai139 transgene (Addgene, #114426) and p21-Myc-Flag from the L-p21 transgene (Origene, #MR227529) as described above. .. Homology arms spanning 968 bp at the 5′-end and 937 bp at the 3′-end flanked by sgRNA target sites were used to target the construct into the Col1a1 locus of C57BL/6NHsd (Envigo) zygotes using CRISPR-Cas9-mediated gene editing with Cas9 mRNA (Trilink Biotechnologies, #L-7606) and Col1a1-specific sgRNA 5′-GAGGTTCATGAGCCCTCAAA-3′.

    Clone Assay:

    Article Title: p21 produces a bioactive secretome that places stressed cells under immunosurveillance
    Article Snippet: .. To generate iL-p21 transgenic mice on a pure C57BL/6 background, the following targeting construct was cloned: pTRE2-LoxP-STOP-LoxP(LSL)-p21-Myc-Flag-WPRE-pA using the pTRE2 promoter and LSL from the Ai139 transgene (Addgene, #114426) and p21-Myc-Flag from the L-p21 transgene (Origene, #MR227529) as described above. .. Homology arms spanning 968 bp at the 5′-end and 937 bp at the 3′-end flanked by sgRNA target sites were used to target the construct into the Col1a1 locus of C57BL/6NHsd (Envigo) zygotes using CRISPR-Cas9-mediated gene editing with Cas9 mRNA (Trilink Biotechnologies, #L-7606) and Col1a1 -specific sgRNA 5′-GAGGTTCATGAGCCCTCAAA-3′.

    Article Title: p21 produces a bioactive secretome that places stressed cells under immunosurveillance.
    Article Snippet: .. To generate iL-p21 transgenic mice on a pure C57BL/6 background, the following targeting construct was cloned: pTRE2-LoxP-STOP-LoxP(LSL)-p21Myc-Flag-WPRE-pA using the TRE2 promoter and LSL from the Ai139 transgene (Addgene, #114426) and p21-Myc-Flag from the L-p21 transgene (Origene, #MR227529) as described above. .. Homology arms spanning 968 bp at the 5′-end and 937 bp at the 3′-end flanked by sgRNA target sites were used to target the construct into the Col1a1 locus of C57BL/6NHsd (Envigo) zygotes using CRISPR-Cas9-mediated gene editing with Cas9 mRNA (Trilink Biotechnologies, #L-7606) and Col1a1-specific sgRNA 5′-GAGGTTCATGAGCCCTCAAA-3′.



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    ai139 transgene  (Addgene inc)
    93
    Addgene inc ai139 transgene
    Ai139 Transgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ai139+transgene/Ai139+(TIT2L-GFP-ICL-TPT)+targeting+vector+(Plasmid+%23114426)/pmc08985214-612-25-27
    Average 93 stars, based on 1 article reviews
    ai139 transgene - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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